M Protein Gene (emm) Typing of Streptococcus pyogenes
نویسندگان
چکیده
منابع مشابه
emm gene polymorphism among streptococcus pyogenes isolated from
dna sequencing is the most conclusive method for emm (m protein gene) typing of streptococcus pyogenes. this method is not a feasible approach in developing countries where streptococcal infection is widespread among adults and children. alternatively the pcr-rflp has the potential for rapid screening of different types of s. pyogenes. to document the emm type distribution of s. pyogenes in a g...
متن کاملEffect of M protein of Streptococcus pyogenes on macrophage functions.
Streptococcal M protein was studied to ascertain its effect on macrophage functions such as oxidative metabolism, phagocytosis, and microbicidal activity. No significant stimulating activity of M protein against macrophage functions such as H 20 2 or 0 2 releasing ability in response to phorbol myristate acetate triggering was noted. M protein did not enhance the macrophage-mediated host risist...
متن کاملEpidemiological Study of Erythromycin-Resistant Streptococcus pyogenes From Korea and Japan by emm Genotyping and Multilocus Sequence Typing
BACKGROUND We determined the epidemiological characteristics of erythromycin (EM)-resistant Streptococcus pyogenes (group A streptococci, GAS) strains isolated from Korea and Japan, using emm genotyping and multilocus sequence typing (MLST). METHODS Clinical isolates of GAS had been collected from 1992 to 2012 in Korea and from 2004 to 2009 in Japan. EM resistance was determined by the microd...
متن کاملMultilocus sequence typing of Streptococcus pyogenes representing most known emm types and distinctions among subpopulation genetic structures.
A long-term goal is to characterize the full range of genetic diversity within Streptococcus pyogenes as it exists in the world today. Since the emm locus is subject to strong diversifying selection, emm type was used as a guide for identifying a genetically diverse set of strains. This report contains a description of multilocus sequence typing based on seven housekeeping loci for 495 isolates...
متن کاملTyping of Streptococcus pyogenes strains using the phage profiling method
We recently developed a method that allows fast differentiation between Streptococcus pyogenes (GAS) strains. The method named phage profiling (PP) is based on a simple assumption that a regular PCR reaction with Taq polymerase and relatively short elongation time is not able to yield long DNA fragment, such as ~40-50 kb integrated prophage. Only fragments without any integrated DNA or short fr...
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ژورنال
عنوان ژورنال: Journal of the Japanese Association for Infectious Diseases
سال: 2002
ISSN: 1884-569X,0387-5911
DOI: 10.11150/kansenshogakuzasshi1970.76.238